The implementation of the Information Systems (IS) Architecture is one of the steps in the construction of the Enterprise Architecture (EA), according to The Open Group Architecture Framework (TOGAF), and can bring notable benefits in supporting the management and governance of Information and Communication Technology (ICT) in organizations, including of health. However, this task is usually followed by several challenges, even more so when we refer to University Hospitals (HU), which are hospitals dedicated to teaching, research, and extension. The purpose of this book is to disseminate an empirical research whose methodological course was carried out in three phases: 1- an extensive Systematic Literature Review, based on 9 specific research questions, to explore the state of the art of the application of EA in health. 2- proposed the 5tep Model, with five well-defined steps to help the implementation of the IS Architecture in HUs. 3- practical application of the 5tep Model together with TOGAF 9.2 and the Archi tool to build and deliver the IS Architecture of a large HU which seeks to improve the provision of services to the population through investments in ICT. As result, in addition to relevant data to the literature on EA and IS through the SLR and the 5tep Model, the delivered IS Architecture for this UH offers a holistic view of the entire ICT, in which identified problems, challenges, possible solutions, and critical success factors for the hospital business.